ABSTRACT

A disposable blood sampler, which is consisted of a sub-microliter whole blood collector and a graphene filter, loading graphene sheet to selectively capture hemoglobin from sub-microliter whole blood, was developed for both qualitative and quantitative characterization hemoglobin by internal extractive electrospray ionization mass spectrometry (iEESI-MS). The blood collector was elegantly fabricated in syringe-like fashion for precisely sampling tiny amounts (1.0 μL-2%) of whole blood, which was immediately diluted by water inside the syringe and was then pressed through the graphene filter placed between the waste outlet and the syringe reservoir to capture the hemoglobin in the blood sample. Then the graphene with hemoglobin was directly eluted by a charged (+2.5 kV) solution (mathanol/water/formic acid, 48/48/4, v/v/v) to produce the hemoglobin ions for mass spectrometric analysis. Low detection-of-limit (19.3 mg/L (89.5 picomol)), acceptable linear response range (300–1500 mg/L, R2=0.998), relative standard deviation (0.5–6.5%, n=3), low sample consumption (≤1.0 μL) and a relatively high speed (≤4 min per sample, including the sample loading) were achieved, demonstrating that the graphene based iEESI-MS was an alternative choice for direct detection of hemoglobin in whole blood with minimal sample consumption.

2019-宋丽丽-Talanta-SCI.pdf