Abstract: A new single labelled DNAzyme sensor was developed for trace UO₂²⁺detection based on the fact that the four consecutive guanines at the 5′-end of DNAzyme quenches the fluorescence of fluophor-tetramethyl-6-carboxyrhodamine (TAMRA) modified at the 3′-end of substrate. When UO₂²⁺coexisted with DNAzyme sensor, UO₂²⁺catalyzed the phosphoester cleavage reaction at the rA position of the substrate accompanied by the fluorescence emission significantly increased. The detection limit of the new DNAzyme sensor is 0.41 nmol/L, which is sensitive enough for UO₂²⁺detection in drinking water as the toxic UO₂²⁺level in drinking water defined by the U.S. Environmental Protection Agency (EPA) is 130 nM. Moreover, the new DNAzyme sensor could discriminate the ores with and without UO₂²⁺, and the exact content of trace UO₂²⁺in ores and natural water samples could also be quantified. In the foreseeable future, the new DNAzyme sensor will be extensively applicable in field environmental monitoring, uranium mining and process control in virtue of portable fluorometers.

Keywords: DNAzyme sensor; UO₂²⁺; Guanine quenching; Ore samples; Natural water samples

2015.1-肖赛金-Sensors and acutators B.pdf