Abstract: Clinically obtained human kidney stones of different pathogenesis were dissolved in acetic acid/methanol solutions and then rapidly analyzed by surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS)without any desalination treatment. The mass spectral fingerprints of six groups of kidney stone sampleswere rapidly recorded in themass range ofm/z 50–400.Aset of tenmelamine-induced kidney stone samples and nine uric acid derived kidney stone sampleswere successfully differentiated from other groups by principal component analysis of SDAPCI-MS fingerprints upon positive-ion detectionmode. In contrast, the mass spectra recorded using negative-ion detectionmode did not give enough information to differentiate those stone samples. The results showed that in addition to the melamine, the chemical compounds enwrapped in the melamine-induced kidney stone samples differed from other kidney stone samples, providing useful hints for studying on the formation mechanisms of melamine-induced kidney stones. This study also provides useful information on establishing a MS-based platform for rapid analysis of the melamine-induced human kidney stones at molecular levels. Copyright_c 2011 John Wiley & Sons, Ltd.

Keywords: surface desorption atmospheric pressure chemical ionizationmass spectrometry; kidney stone; melamine; uric acid; principal component analysis

2011-贾斌-J. Mass Spectrom.,.pdf