Direct mass spectrometry analysis of metabolic effects of clenbuterol and salbutamol on pork quality
at the molecular level is incredibly beneficial for food regulations, public health and the development of
new anti-obesity drugs. With internal extractive electrospray ionization mass spectrometry (iEESI-MS),
nutrients including creatine, amino acids, L-carnitine, vitamin B6, carnosine and phosphatidylcholines
in pork tissue were identified, without sample pretreatment, using collision-induced dissociation (CID)
experiments and by comparison with authentic compounds. Furthermore, normal pork samples were
clearly differentiated from pork samples with clenbuterol and salbutamol via principal component
analysis (PCA). Correlation analysis performed on the spectral data revealed that the above-mentioned
nutrients strongly correlated with pork quality, and the absolute intensity of phosphatidylcholines
in normal pork was much higher than pork contaminated by clenbuterol and salbutamol. Our
findings suggested that clenbuterol and salbutamol may render effects on the activity of carnitine
acyltransferase I, hence the process that L-carnitine transports long-chain fatty acids into mitochondria
and the formation of phosphatidylcholines might be affected. However, the underlying metabolic
mechanisms of clenbuterol and salbutamol on carnitine acyltransferase I requires more comprehensive
studies in future work.

2017-卢海艳-Scienfitic Reports-Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry.pdf